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Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins.

ABSTRACT: Thioamides are single-atom substitutions of canonical amide bonds, and have been proven to be versatile and minimally perturbing probes in protein folding studies. Previously, our group showed that thioamides can be incorporated into proteins by native chemical ligation (NCL) with Cys as a ligation handle. In this study, we report the expansion of this strategy into non-Cys ligation sites, utilizing radical initiated desulfurization to "erase" the side chain thiol after ligation. The reaction exhibited high chemoselectivity against thioamides, which can be further enhanced with thioacetamide as a sacrificial scavenger. As a proof-of-concept example, we demonstrated the incorporation of a thioamide probe into a 56 amino acid protein, the B1 domain of Protein G (GB1). Finally, we showed that the method can be extended to ?-thiol amino acid analogs and selenocysteine.

SUBMITTER: Wang YJ

PROVIDER: S-EPMC4927387 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins.

Wang Yanxin J YJ Szantai-Kis D Miklos DM Petersson E James EJ

Organic & biomolecular chemistry 20160606 26

Thioamides are single-atom substitutions of canonical amide bonds, and have been proven to be versatile and minimally perturbing probes in protein folding studies. Previously, our group showed that thioamides can be incorporated into proteins by native chemical ligation (NCL) with Cys as a ligation handle. In this study, we report the expansion of this strategy into non-Cys ligation sites, utilizing radical initiated desulfurization to "erase" the side chain thiol after ligation. The reaction ex ...[more]

PMID: 27264841

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