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ABSTRACT:
SUBMITTER: Dippel AB
PROVIDER: S-EPMC4756619 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Acta crystallographica. Section D, Structural biology 20160101 Pt 1
The X-ray crystal structures of superfolder green fluorescent protein (sfGFP) containing the spectroscopic reporter unnatural amino acids (UAAs) 4-cyano-L-phenylalanine (pCNF) or 4-ethynyl-L-phenylalanine (pCCF) at two unique sites in the protein have been determined. These UAAs were genetically incorporated into sfGFP in a solvent-exposed loop region and/or a partially buried site on the β-barrel of the protein. The crystal structures containing the UAAs at these two sites permit the structural ...[more]