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Probing the role of the cation-pi interaction in the binding sites of GPCRs using unnatural amino acids.


ABSTRACT: We describe a general application of the nonsense suppression methodology for unnatural amino acid incorporation to probe drug-receptor interactions in functional G protein-coupled receptors (GPCRs), evaluating the binding sites of both the M2 muscarinic acetylcholine receptor and the D2 dopamine receptor. Receptors were expressed in Xenopus oocytes, and activation of a G protein-coupled, inward-rectifying K(+) channel (GIRK) provided, after optimization of conditions, a quantitative readout of receptor function. A number of aromatic amino acids thought to be near the agonist-binding site were evaluated. Incorporation of a series of fluorinated tryptophan derivatives at W6.48 of the D2 receptor establishes a cation-pi interaction between the agonist dopamine and W6.48, suggesting a reorientation of W6.48 on agonist binding, consistent with proposed "rotamer switch" models. Interestingly, no comparable cation-pi interaction was found at the aligning residue in the M2 receptor.

SUBMITTER: Torrice MM 

PROVIDER: S-EPMC2715518 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Probing the role of the cation-pi interaction in the binding sites of GPCRs using unnatural amino acids.

Torrice Michael M MM   Bower Kiowa S KS   Lester Henry A HA   Dougherty Dennis A DA  

Proceedings of the National Academy of Sciences of the United States of America 20090706 29


We describe a general application of the nonsense suppression methodology for unnatural amino acid incorporation to probe drug-receptor interactions in functional G protein-coupled receptors (GPCRs), evaluating the binding sites of both the M2 muscarinic acetylcholine receptor and the D2 dopamine receptor. Receptors were expressed in Xenopus oocytes, and activation of a G protein-coupled, inward-rectifying K(+) channel (GIRK) provided, after optimization of conditions, a quantitative readout of  ...[more]

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