Ontology highlight
ABSTRACT:
SUBMITTER: Torrice MM
PROVIDER: S-EPMC2715518 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Torrice Michael M MM Bower Kiowa S KS Lester Henry A HA Dougherty Dennis A DA
Proceedings of the National Academy of Sciences of the United States of America 20090706 29
We describe a general application of the nonsense suppression methodology for unnatural amino acid incorporation to probe drug-receptor interactions in functional G protein-coupled receptors (GPCRs), evaluating the binding sites of both the M2 muscarinic acetylcholine receptor and the D2 dopamine receptor. Receptors were expressed in Xenopus oocytes, and activation of a G protein-coupled, inward-rectifying K(+) channel (GIRK) provided, after optimization of conditions, a quantitative readout of ...[more]