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Alternative splicing creates two new architectures for human tyrosyl-tRNA synthetase.


ABSTRACT: Many human tRNA synthetases evolved alternative functions outside of protein synthesis. These functions are associated with over 200 splice variants (SVs), most of which are catalytic nulls that engender new biology. While known to regulate non-translational activities, little is known about structures resulting from natural internal ablations of any protein. Here, we report analysis of two closely related, internally deleted, SVs of homodimeric human tyrosyl-tRNA synthetase (TyrRS). In spite of both variants ablating a portion of the catalytic core and dimer-interface contacts of native TyrRS, each folded into a distinct stable structure. Biochemical and nuclear magnetic resonance (NMR) analysis showed that the internal deletion of TyrRS?E2-4 SV gave an alternative, neomorphic dimer interface 'orthogonal' to that of native TyrRS. In contrast, the internal C-terminal splice site of TyrRS?E2-3 prevented either dimerization interface from forming, and yielded a predominantly monomeric protein. Unlike ubiquitous TyrRS, the neomorphs showed clear tissue preferences, which were distinct from each other. The results demonstrate a sophisticated structural plasticity of a human tRNA synthetase for architectural reorganizations that are preferentially elicited in specific tissues.

SUBMITTER: Wei Z 

PROVIDER: S-EPMC4756856 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Alternative splicing creates two new architectures for human tyrosyl-tRNA synthetase.

Wei Zhiyi Z   Xu Zhiwen Z   Liu Xiaotian X   Lo Wing-Sze WS   Ye Fei F   Lau Ching-Fun CF   Wang Feng F   Zhou Jie J JJ   Nangle Leslie A LA   Yang Xiang-Lei XL   Zhang Mingjie M   Schimmel Paul P  

Nucleic acids research 20160114 3


Many human tRNA synthetases evolved alternative functions outside of protein synthesis. These functions are associated with over 200 splice variants (SVs), most of which are catalytic nulls that engender new biology. While known to regulate non-translational activities, little is known about structures resulting from natural internal ablations of any protein. Here, we report analysis of two closely related, internally deleted, SVs of homodimeric human tyrosyl-tRNA synthetase (TyrRS). In spite of  ...[more]

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