Ontology highlight
ABSTRACT:
SUBMITTER: Jones ML
PROVIDER: S-EPMC4759600 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Jones Matthew L ML Das Sujaan S Belda Hugo H Collins Christine R CR Blackman Michael J MJ Treeck Moritz M
Scientific reports 20160219
Conditional genome engineering in the human malaria pathogen Plasmodium falciparum remains highly challenging. Here we describe a strategy for facile and rapid functional analysis of genes using an approach based on the Cre/lox system and tailored for organisms with short and few introns. Our method allows the conditional, site-specific removal of genomic sequences of essential and non-essential genes by placing loxP sites into a short synthetic intron to produce a module (loxPint) can be placed ...[more]