Ontology highlight
ABSTRACT:
SUBMITTER: Burg L
PROVIDER: S-EPMC6261631 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Burg Leonard L Palmer Nicholas N Kikhi Khrievono K Miroshnik Evgeniya S ES Rueckert Helen H Gaddy Eleanor E MacPherson Cunningham Carlee C Mattonet Kenny K Lai Shih-Lei SL Marín-Juez Rubén R Waring Richard B RB Stainier Didier Y R DYR Balciunas Darius D
PLoS genetics 20181114 11
Many eukaryotic genes play essential roles in multiple biological processes in several different tissues. Conditional mutants are needed to analyze genes with such pleiotropic functions. In vertebrates, conditional gene inactivation has only been feasible in the mouse, leaving other model systems to rely on surrogate experimental approaches such as overexpression of dominant negative proteins and antisense-based tools. Here, we have developed a simple and straightforward method to integrate loxP ...[more]