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Size-selective separation of DNA fragments by using lysine-functionalized silica particles.

ABSTRACT: In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and salt concentration, therefore, the use of the lysine-functionalized silica particles allows effective separation of binary and ternary DNA mixtures, for example, two different DNA fragments with sizes of 101 and 1073?bp, 101 and 745?bp, 101 and 408?bp, respectively, and three different DNA fragments with sizes of 101, 408 and 1073?bp.

SUBMITTER: Liu L

PROVIDER: S-EPMC4766563 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Size-selective separation of DNA fragments by using lysine-functionalized silica particles.

Liu Lingling L Guo Zilong Z Huang Zhenzhen Z Zhuang Jiaqi J Yang Wensheng W

Scientific reports 20160225

In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and ...[more]

PMID: 26911527

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