Ontology highlight
ABSTRACT:
SUBMITTER: Khantwal CM
PROVIDER: S-EPMC4769167 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
eLife 20160122
CLC secondary active transporters exchange Cl(-) for H(+). Crystal structures have suggested that the conformational change from occluded to outward-facing states is unusually simple, involving only the rotation of a conserved glutamate (Gluex) upon its protonation. Using (19)F NMR, we show that as [H(+)] is increased to protonate Gluex and enrich the outward-facing state, a residue ~20 Å away from Gluex, near the subunit interface, moves from buried to solvent-exposed. Consistent with functiona ...[more]