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Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.

ABSTRACT: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of ?=500-630?nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1??m solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60?nm.

SUBMITTER: Butkevich AN

PROVIDER: S-EPMC4770443 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells.

Butkevich Alexey N AN Mitronova Gyuzel Yu GY Sidenstein Sven C SC Klocke Jessica L JL Kamin Dirk D Meineke Dirk N H DN D'Este Elisa E Kraemer Philip-Tobias PT Danzl Johann G JG Belov Vladimir N VN Hell Stefan W SW

Angewandte Chemie (International ed. in English) 20160204 10

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-c ...[more]

PMID: 26844929

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