Unknown

Dataset Information

0

IL-22 regulation of functional gene expression in salivary gland cells.


ABSTRACT: TH17 cells and their associated signature cytokines, IL-17 and IL-22, are highly elevated in primary Sjögren's syndrome (pSjS). The levels of IL-22 present in sera showed significant correlations with many disease parameters, specifically hyposalivation, anti-SSB, anti-SSA/SSB, hypergammaglobulinemia and rheumatoid factor. The present study aims to examine the biological function of IL-22 on human salivary glands. To accomplish the goal, microarray analysis using the HumanHT-12 v4 Expression BeadChip was utilized to determine the biological function of IL-22. Differential expression analyses were conducted using the LIMMA package from the Bioconductor project. MTT assay, flow cytometry and Western blotting were used to identify the function of IL-22 on human salivary gland cells. Results indicate an extensive effect of IL-22 on many major molecular functions including activation of antimicrobial genes and downregulation of immune-associated pathways. Functional studies performed in-vitro using human salivary gland cells treated with IL-22 indicated a direct effect of IL-22 on cell cycling, specifically reducing cellular proliferation at the G2-M phase by activation of STAT3. These results suggest the important role of IL-22 in the salivary gland function. The present study suggests that IL-22 might be involved in regulating inflammation and controlling the cell proliferation in SjS.

SUBMITTER: Lavoie TN 

PROVIDER: S-EPMC4778602 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

IL-22 regulation of functional gene expression in salivary gland cells.

Lavoie Tegan N TN   Carcamo Wendy C WC   Wanchoo Arun A   Sharma Ashok A   Gulec Afife A   Berg Kathleen M KM   Stewart Carol M CM   Nguyen Cuong Q CQ  

Genomics data 20151122


TH17 cells and their associated signature cytokines, IL-17 and IL-22, are highly elevated in primary Sjögren's syndrome (pSjS). The levels of IL-22 present in sera showed significant correlations with many disease parameters, specifically hyposalivation, anti-SSB, anti-SSA/SSB, hypergammaglobulinemia and rheumatoid factor. The present study aims to examine the biological function of IL-22 on human salivary glands. To accomplish the goal, microarray analysis using the HumanHT-12 v4 Expression Bea  ...[more]

Similar Datasets

2015-10-27 | GSE74389 | GEO
2015-10-27 | E-GEOD-74389 | biostudies-arrayexpress
| S-EPMC7083056 | biostudies-literature
| S-EPMC4620551 | biostudies-literature
| S-EPMC6181987 | biostudies-literature
| S-EPMC8634938 | biostudies-literature
| S-EPMC5496813 | biostudies-literature
| S-EPMC2263065 | biostudies-literature
| S-EPMC4264052 | biostudies-literature
| S-EPMC4824453 | biostudies-literature