Unknown

Dataset Information

0

The Wnt/?-catenin signaling/Id2 cascade mediates the effects of hypoxia on the hierarchy of colorectal-cancer stem cells.


ABSTRACT: Hypoxia, a feature common to most solid tumors, is known to regulate many aspects of tumorigenesis. Recently, it was suggested that hypoxia increased the size of the cancer stem-cell (CSC) subpopulations and promoted the acquisition of a CSC-like phenotype. However, candidate hypoxia-regulated mediators specifically relevant to the stemness-related functions of colorectal CSCs have not been examined in detail. In the present study, we showed that hypoxia specifically promoted the self-renewal potential of CSCs. Through various in vitro studies, we found that hypoxia-induced Wnt/?-catenin signaling increased the occurrence of CSC-like phenotypes and the level of Id2 expression in colorectal-cancer cells. Importantly, the levels of hypoxia-induced CSC-sphere formation and Id2 expression were successfully attenuated by treatment with a Wnt/?-catenin-signaling inhibitor. We further demonstrated, for the first time, that the degree of hypoxia-induced CSC-sphere formation (CD44(+) subpopulation) in vitro and of tumor metastasis/dissemination in vivo were markedly suppressed by knocking down Id2 expression. Taken together, these data suggested that Wnt/?-catenin signaling mediated the hypoxia-induced self-renewal potential of colorectal-cancer CSCs through reactivating Id2 expression.

SUBMITTER: Dong HJ 

PROVIDER: S-EPMC4786801 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Wnt/β-catenin signaling/Id2 cascade mediates the effects of hypoxia on the hierarchy of colorectal-cancer stem cells.

Dong Hye-Jin HJ   Jang Gyu-Beom GB   Lee Hwa-Yong HY   Park Se-Ra SR   Kim Ji-Young JY   Nam Jeong-Seok JS   Hong In-Sun IS  

Scientific reports 20160311


Hypoxia, a feature common to most solid tumors, is known to regulate many aspects of tumorigenesis. Recently, it was suggested that hypoxia increased the size of the cancer stem-cell (CSC) subpopulations and promoted the acquisition of a CSC-like phenotype. However, candidate hypoxia-regulated mediators specifically relevant to the stemness-related functions of colorectal CSCs have not been examined in detail. In the present study, we showed that hypoxia specifically promoted the self-renewal po  ...[more]

Similar Datasets

| S-EPMC5190041 | biostudies-literature
| S-EPMC8183571 | biostudies-literature
2017-05-01 | GSE97543 | GEO
| S-EPMC8770385 | biostudies-literature
| S-EPMC4741928 | biostudies-literature
| S-EPMC6935018 | biostudies-literature
| S-EPMC5414094 | biostudies-literature
| S-EPMC5731912 | biostudies-literature
| S-EPMC9665845 | biostudies-literature
| S-EPMC1766434 | biostudies-literature