Ontology highlight
ABSTRACT:
SUBMITTER: Reddy Chichili VP
PROVIDER: S-EPMC4789767 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Reddy Chichili Vishnu Priyanka VP Kumar Veerendra V Sivaraman J J
Journal of biological methods 20160101 1
Protein-protein interactions are key events controlling several biological processes. We have developed and employed a method to trap transiently interacting protein complexes for structural studies using glycine-rich linkers to fuse interacting partners, one of which is unstructured. Initial steps involve isothermal titration calorimetry to identify the minimum binding region of the unstructured protein in its interaction with its stable binding partner. This is followed by computational analys ...[more]