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Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy.

ABSTRACT: N-terminal sequences play crucial roles in regulating expression, translation, activation and enzymatic properties of proteins. To reduce cell toxicity of intracellular trypsin and increase secretory expression, we developed a novel auto-catalyzed strategy to produce recombinant trypsin by engineering the N-terminus of mature Streptomyces griseus trypsin (SGT). The engineered N-terminal peptide of SGT was composed of the thioredoxin, glycine-serine linker, His6-tag and the partial bovine trypsinogen pro-peptide (DDDDK). Furthermore, we constructed a variant TLEI with insertion of the artificial peptide at N-terminus and site-directed mutagenesis of the autolysis residue R145. In fed-batch fermentation, the production of extracellular trypsin activity was significantly improved to 47.4?±?1.2?U·ml(-1) (amidase activity, 8532?±?142.2?U·ml(-1) BAEE activity) with a productivity of 0.49?U·ml(-1)·h(-1), which was 329% greater than that of parent strain Pichia pastoris GS115-SGT. This work has significant potential to be scaled-up for microbial production of SGT. In addition, the N-terminal peptide engineering strategy can be extended to improve heterologous expression of other toxic enzymes.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC4794721 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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