Ontology highlight
ABSTRACT:
SUBMITTER: Hoshijima K
PROVIDER: S-EPMC4806538 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Hoshijima Kazuyuki K Jurynec Michael J MJ Grunwald David Jonah DJ
Developmental cell 20160301 6
We present simple and efficient methods for creating heritable modifications of the zebrafish genome. Precisely modified alleles are generated by homologous recombination between the host genome and dsDNA donor molecules, stimulated by the induction of chromosomally targeted double-strand breaks. Several kilobase-long tracts of genome sequence can be replaced. Tagging donor sequences with reporter genes that can be subsequently excised improves recovery of edited alleles by an order of magnitude ...[more]