Dataset Information

Multi-Scale Molecular Deconstruction of the Serotonin Neuron System.

ABSTRACT: Serotonergic (5HT) neurons modulate diverse behaviors and physiology and are implicated in distinct clinical disorders. Corresponding diversity in 5HT neuronal phenotypes is becoming apparent and is likely rooted in molecular differences, yet a comprehensive approach characterizing molecular variation across the 5HT system is lacking, as is concomitant linkage to cellular phenotypes. Here we combine intersectional fate mapping, neuron sorting, and genome-wide RNA-seq to deconstruct the mouse 5HT system at multiple levels of granularity-from anatomy, to genetic sublineages, to single neurons. Our unbiased analyses reveal principles underlying system organization, 5HT neuron subtypes, constellations of differentially expressed genes distinguishing subtypes, and predictions of subtype-specific functions. Using electrophysiology, subtype-specific neuron silencing, and conditional gene knockout, we show that these molecularly defined 5HT neuron subtypes are functionally distinct. Collectively, this resource classifies molecular diversity across the 5HT system and discovers sertonergic subtypes, markers, organizing principles, and subtype-specific functions with potential disease relevance.

SUBMITTER: Okaty BW

PROVIDER: S-EPMC4809055 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Publications

Multi-Scale Molecular Deconstruction of the Serotonin Neuron System.

Okaty Benjamin W BW Freret Morgan E ME Rood Benjamin D BD Brust Rachael D RD Hennessy Morgan L ML deBairos Danielle D Kim Jun Chul JC Cook Melloni N MN Dymecki Susan M SM

Neuron 20151105 4

Serotonergic (5HT) neurons modulate diverse behaviors and physiology and are implicated in distinct clinical disorders. Corresponding diversity in 5HT neuronal phenotypes is becoming apparent and is likely rooted in molecular differences, yet a comprehensive approach characterizing molecular variation across the 5HT system is lacking, as is concomitant linkage to cellular phenotypes. Here we combine intersectional fate mapping, neuron sorting, and genome-wide RNA-seq to deconstruct the mouse 5HT ...[more]

PMID: 26549332

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Dataset Information

Multi-Scale Molecular Deconstruction of the Serotonin Neuron System.

Publications

Multi-Scale Molecular Deconstruction of the Serotonin Neuron System.

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