Ontology highlight
ABSTRACT:
SUBMITTER: Pineda G
PROVIDER: S-EPMC4819192 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Pineda Gabriel G Lennon Kathleen M KM Delos Santos Nathaniel P NP Lambert-Fliszar Florence F Riso Gennarina L GL Lazzari Elisa E Marra Marco A MA Morris Sheldon S Sakaue-Sawano Asako A Miyawaki Atsushi A Jamieson Catriona H M CH
Scientific reports 20160404
While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia proge ...[more]