Ontology highlight
ABSTRACT:
SUBMITTER: Mehra-Chaudhary R
PROVIDER: S-EPMC4819441 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Mehra-Chaudhary Ritcha R Dai Yumin Y Sobrado Pablo P Tanner John J JJ
Biochemistry 20160204 6
UDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in pathogens by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. Here we report the first crystal structure of a covalent intermediate in the UGM reaction. The 2.3 Å resolution structure reveals UDP bound in the active site and galactopyranose linked to the FAD through a covalent bond between the anomeric C of galactopyranose and N5 of the FAD. The structure confirms the role of the f ...[more]