Dataset Information

Management practices regulate the response of Moso bamboo foliar stoichiometry to nitrogen deposition.

ABSTRACT: Moso bamboo, well known for its high growth rate, is being subjected to increasing amounts of nitrogen deposition. However, how anthropogenic management practices regulate the effects of N deposition on Moso bamboo stoichiometry remains poorly understood. We observed the effects of two years of simulated N deposition (30, 60 and 90 kg N ha(-1)yr(-1)) on the foliar stoichiometry of Moso bamboo plantations under conventional management (CM) and intensive management (IM). Young bamboo had significantly greater foliar N and P concentrations and N:P ratios than mature plants (P < 0.05). IM significantly increased the foliar N concentrations of young bamboo and P concentrations of mature bamboo but decreased mature bamboo foliar N:P ratios (P < 0.05). Nitrogen increased foliar N and P concentrations in IM bamboo plantations, but the positive effects were diminished when the addition rate exceeded 60 kg N ha(-1)yr(-1). Nitrogen increased foliar N concentrations but aggravated P deficiency in CM bamboo plantations. The positive effects of N deposition on foliar stoichiometry were influenced by management practices and bamboo growth stage. The effects of N deposition on foliar stoichiometry combined with anthropogenic management practices can influence ecosystem production, decomposition, and subsequent N and P cycles in Moso bamboo plantations.

SUBMITTER: Song X

PROVIDER: S-EPMC4823648 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Management practices regulate the response of Moso bamboo foliar stoichiometry to nitrogen deposition.

Song Xinzhang X Gu Honghao H Wang Meng M Zhou Guomo G Li Quan Q

Scientific reports 20160407

Moso bamboo, well known for its high growth rate, is being subjected to increasing amounts of nitrogen deposition. However, how anthropogenic management practices regulate the effects of N deposition on Moso bamboo stoichiometry remains poorly understood. We observed the effects of two years of simulated N deposition (30, 60 and 90 kg N ha(-1)yr(-1)) on the foliar stoichiometry of Moso bamboo plantations under conventional management (CM) and intensive management (IM). Young bamboo had significa ...[more]

PMID: 27052002

Similar Datasets

Project description:Atmospheric nitrogen (N) deposition can increase the susceptibility of vascular plants to other stresses, but the physiological basis of such a response remains poorly understood. This study was designed to clarify the physiological mechanisms and to evaluate bioindicators of N deposition impact on vascular plants. We evaluate multiple physiological responses to ~4 years of simulated additional N deposition (30-90 kg N ha-1 year-1) on three age-classes (1a, 3a, and 5a) of Moso bamboo. A saturating response to the additional N deposition was found both in foliar N concentration and in Pn. However, 3- and 5-year-old bamboo seemed to be less tolerant to extremely high N deposition than 1-year-old bamboo since they were saturated at a lower N addition. Furthermore, C/N/P stoichiometric ratios were very sensitive to N deposition in all three-age classes of bamboo, but the responses to N deposition in the various age-classes were diverse. We also found that the highest additional N deposition suppressed stomatal conductance and transpiration rate, suggesting an induced water stress. The stress induced by the high N load was also seen in photochemistry, where it reduced potential and actual photosynthetic use of light energy, diminished photo-protection capacity, and increased risk of the photo-damage. High additional N deposition contributed to a decrease in the foliar soluble protein contents and to an increase in the peroxidase activity (POD). Our study suggested, for the first time, that although the photosynthetic rate was enhanced by the increased N deposition in Moso bamboo, long-term high N load causes negative effects, such as damage to photosystem II. In Moso bamboo photochemical parameters are more sensitive to N deposition than photosynthetic rate or foliar N concentration. Furthermore, plant age should be taken into account when assessing plants' susceptibility to changes in global change drivers, such as N deposition. These findings facilitate the revealing of the risks potentially caused to vascular plants by increased N deposition before any visible symptoms of injury are seen.

Project description:IntroductionConsidering the rapid growth and high biomass productivity, Moso bamboo (Phyllostachys edulis) has high carbon (C) sequestration potential, and different management practices can strongly modify its C pools. Soil microorganisms play an important role in C turnover through dead plant and microbial biomass degradation. To date, little is known about how different management practices affect microbial carbohydrate-active enzymes (CAZymes) and their responses to dead biomass degradation.MethodsBased on metagenomics analysis, this study analyzed CAZymes in three comparable stands from each Moso bamboo plantation: undisturbed (M0), extensively managed (M1), and intensively managed (M2).ResultsThe results showed that the number of CAZymes encoding plant-derived component degradation was higher than that encoding microbe-derived component degradation. Compared with the M0, the CAZyme families encoding plant-derived cellulose were significantly (p < 0.05) high in M2 and significantly (p < 0.05) low in M1. For microbe-derived components, the abundance of CAZymes involved in the bacterial-derived peptidoglycan was higher than that in fungal-derived components (chitin and glucans). Furthermore, M2 significantly increased the fungal-derived chitin and bacterial-derived peptidoglycan compared to M0, whereas M1 significantly decreased the fungal-derived glucans and significantly increased the bacterial-derived peptidoglycan. Four bacterial phyla (Acidobacteria, Actinobacteria, Proteobacteria, and Chloroflexi) mainly contributed to the degradation of C sources from the plant and microbial biomass. Redundancy analysis (RDA) and mantel test suggested the abundance of CAZyme encoding genes for plant and microbial biomass degradation are significantly correlated with soil pH, total P, and available K. Least Squares Path Modeling (PLS-PM) showed that management practices indirectly affect the CAZyme encoding genes associated with plant and microbial biomass degradation by regulating the soil pH and nutrients (total N and P), respectively.DiscussionOur study established that M2 and M1 impact dead biomass decomposition and C turnover, contributing to decreased C accumulation and establishing that the bacterial community plays the main role in C turnover in bamboo plantations.

Project description:Nitrogen is a key macronutrient essential for plant growth and development, and its availability has a strong influence on biological processes. Nitrogen fertilizer has been widely applied in bamboo forests in recent decades; however, the mechanism of nitrogen metabolism in bamboo is not fully elucidated. Here, we characterized the morphological, physiological, and transcriptome changes of moso bamboo in response to different schemes for nitrogen addition to illuminate the regulation mechanism of nitrogen metabolism. The appropriate addition of nitrogen improved the chlorophyll content and Pn (net photosynthetic rate) of leaves, the nitrogen and ammonium contents of the seedling roots, the biomass of the whole seedling, the number of lateral roots, and the activity of enzymes involved in nitrogen metabolism in the roots. Based on the whole transcriptome data of the roots, a total of 8,632 differentially expressed mRNAs (DEGs) were identified under different nitrogen additions, such as 52 nitrate transporter genes, 6 nitrate reductase genes, 2 nitrite reductase genes, 2 glutamine synthase genes, 2 glutamate synthase genes (GOGAT), 3 glutamate dehydrogenase genes, and 431 TFs belonging to 23 families. Meanwhile, 123 differentially expressed miRNAs (DEMs) and 396 differentially expressed lncRNAs (DELs) were characterized as nitrogen responsive, respectively. Furthermore, 94 DEM-DEG pairs and 23 DEL-DEG pairs involved in nitrogen metabolism were identified. Finally, a predicted regulatory network of nitrogen metabolism was initially constructed, which included 17 nitrogen metabolic pathway genes, 15 TFs, 4 miRNAs, and 10 lncRNAs by conjoint analysis of DEGs, DEMs, and DELs and their regulatory relationships, which was supported by RNA-seq data and qPCR results. The lncRNA-miRNA-mRNA network provides new insights into the regulation mechanism of nitrogen metabolism in bamboo, which facilitates further genetic improvement for bamboo to adapt to the fluctuating nitrogen environment.

Dataset Information

Management practices regulate the response of Moso bamboo foliar stoichiometry to nitrogen deposition.

Publications

Management practices regulate the response of Moso bamboo foliar stoichiometry to nitrogen deposition.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets