Dataset Information

Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest.

ABSTRACT: Forest soils play an important role in controlling global warming by reducing atmospheric methane (CH₄) concentrations. However, little attention has been paid to how nitrogen (N) deposition may alter microorganism communities that are related to the CH₄ cycle or CH₄ oxidation in subtropical forest soils. We investigated the effects of N addition (0, 30, 60, or 90 kg N ha^-1 yr^-1) on soil CH₄ flux and methanotroph and methanogen abundance, diversity, and community structure in a Moso bamboo (Phyllostachys edulis) forest in subtropical China. N addition significantly increased methanogen abundance but reduced both methanotroph and methanogen diversity. Methanotroph and methanogen community structures under the N deposition treatments were significantly different from those of the control. In N deposition treatments, the relative abundance of Methanoculleus was significantly lower than that in the control. Soil pH was the key factor regulating the changes in methanotroph and methanogen diversity and community structure. The CH₄ emission rate increased with N addition and was negatively correlated with both methanotroph and methanogen diversity but positively correlated with methanogen abundance. Overall, our results suggested that N deposition can suppress CH₄ uptake by altering methanotroph and methanogen abundance, diversity, and community structure in subtropical Moso bamboo forest soils.

SUBMITTER: Li Q

PROVIDER: S-EPMC7947007 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest.

Li Quan Q Peng Changhui C Zhang Junbo J Li Yongfu Y Song Xinzhang X

Scientific reports 20210310 1

Forest soils play an important role in controlling global warming by reducing atmospheric methane (CH4) concentrations. However, little attention has been paid to how nitrogen (N) deposition may alter microorganism communities that are related to the CH4 cycle or CH4 oxidation in subtropical forest soils. We investigated the effects of N addition (0, 30, 60, or 90 kg N ha-1 yr-1) on soil CH4 flux and methanotroph and methanogen ab ...[more]

PMID: 33692387

Similar Datasets

Project description:Atmospheric nitrogen (N) deposition can increase the susceptibility of vascular plants to other stresses, but the physiological basis of such a response remains poorly understood. This study was designed to clarify the physiological mechanisms and to evaluate bioindicators of N deposition impact on vascular plants. We evaluate multiple physiological responses to ~4 years of simulated additional N deposition (30-90 kg N ha-1 year-1) on three age-classes (1a, 3a, and 5a) of Moso bamboo. A saturating response to the additional N deposition was found both in foliar N concentration and in Pn. However, 3- and 5-year-old bamboo seemed to be less tolerant to extremely high N deposition than 1-year-old bamboo since they were saturated at a lower N addition. Furthermore, C/N/P stoichiometric ratios were very sensitive to N deposition in all three-age classes of bamboo, but the responses to N deposition in the various age-classes were diverse. We also found that the highest additional N deposition suppressed stomatal conductance and transpiration rate, suggesting an induced water stress. The stress induced by the high N load was also seen in photochemistry, where it reduced potential and actual photosynthetic use of light energy, diminished photo-protection capacity, and increased risk of the photo-damage. High additional N deposition contributed to a decrease in the foliar soluble protein contents and to an increase in the peroxidase activity (POD). Our study suggested, for the first time, that although the photosynthetic rate was enhanced by the increased N deposition in Moso bamboo, long-term high N load causes negative effects, such as damage to photosystem II. In Moso bamboo photochemical parameters are more sensitive to N deposition than photosynthetic rate or foliar N concentration. Furthermore, plant age should be taken into account when assessing plants' susceptibility to changes in global change drivers, such as N deposition. These findings facilitate the revealing of the risks potentially caused to vascular plants by increased N deposition before any visible symptoms of injury are seen.

Project description:BackgroundThis study aimed to gain an understanding of the growth response of Phyllostachys edulis (moso bamboo) seedlings to nitrogen (N) and potassium (K) to benefit nutrient management practices and the design of proper fertilizer in nursery cultivation.MethodsAn orthogonal array L8(4×24) was used to study the effects of N forms (NH4 +, NO3 -), N concentrations (8, 32 mmol/L), and K+concentrations (0, 0.5, 1.5, 3 mmol/L) on seedling height, leaf number, chlorophyll content (SPAD value), biomass, root systems, and N content of P. edulis seedlings. Plants were grown in vermiculite under controlled greenhouse conditions.ResultsOur study showed that N form played a significant role in the overall performance of P. edulis seedlings, followed by the effect of N and K+ concentrations. Among the N forms, NH4 + significantly improved the growth of P. edulis seedlings compared with NO3 -. Seedling height, leaf number, chlorophyll SPAD value, biomass, and root system architecture (root length, root surface area, root volume, and root tips) were greater with 8 mmol/L of NH4 + treatments than with 32 mmol/L of NH4 +treatments, whereas root diameter and N content of P. edulis seedlings were higher with 32 mmol/L of NH4 + than with 8 mmol/L of NH4 +. K displayed inconsistent effects on the growth of P. edulis seedlings. Specifically, seedling height, leaf number, biomass and root volume increased when the K+ concentration was increased from 0 to 0.5 mmol/L, followed by a decrease when the K+ concentration was further increased from 0.5 to 3 mmol/L. Root average diameter of the seedlings was the highest with a K+ concentration of 1.5 mmol/L, and K had some inhibitory effects on the chlorophyll SPAD value of the seedlings. P. edulis seedlings performed well with 8 mmol/L NH4 +and further tolerated a higher concentration of both NH4 + and NO3 -, although excessive N could inhibit seedling growth. A lower concertation of K (≤ 0.5 mmol/L) promoted seedling growth and increasing K+ concentration in the nutrient solution did not alleviate the inhibitory effect of high N on the growth of P. edulis seedlings. Therefore, NH4 +nitrogen as the main form of N fertilizer, together with a low concertation of K+, should be supplied in the cultivation and nutrient management practices of moso bamboo.

Dataset Information

Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest.

Publications

Nitrogen addition decreases methane uptake caused by methanotroph and methanogen imbalances in a Moso bamboo forest.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets