Unknown

Dataset Information

0

A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-?-Lactamase.


ABSTRACT: A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-?-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniBdelta-tniA. The isolate belonged to multilocus sequence typing ST235, one of the international high-risk clones. IMP-34, with an amino acid substitution (Glu126Gly) compared with IMP-1, hydrolyzed all ?-lactamases tested except aztreonam, and its catalytic activities were similar to IMP-1. This is the first report of a clinical isolate of an IMP-34-producing P. aeruginosa harboring two copies of blaIMP-34 on its chromosome.

SUBMITTER: Tada T 

PROVIDER: S-EPMC4824433 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Carbapenem-Resistant Pseudomonas aeruginosa Isolate Harboring Two Copies of blaIMP-34 Encoding a Metallo-β-Lactamase.

Tada Tatsuya T   Miyoshi-Akiyama Tohru T   Shimada Kayo K   Shiroma Akino A   Nakano Kazuma K   Teruya Kuniko K   Satou Kazuhito K   Hirano Takashi T   Shimojima Masahiro M   Kirikae Teruo T  

PloS one 20160407 4


A carbapenem-resistant strain of Pseudomonas aeruginosa, NCGM1984, was isolated in 2012 from a hospitalized patient in Japan. Immunochromatographic assay showed that the isolate was positive for IMP-type metallo-β-lactamase. Complete genome sequencing revealed that NCGM1984 harbored two copies of blaIMP-34, located at different sites on the chromosome. Each blaIMP-34 was present in the same structures of the class 1 integrons, tnpA(ISPa7)-intI1-qacG-blaIMP-34-aac(6')-Ib-qacEdelta1-sul1-orf5-tniB  ...[more]

Similar Datasets

| S-EPMC6609741 | biostudies-literature
| S-EPMC3020596 | biostudies-literature
| S-EPMC514723 | biostudies-literature
| S-EPMC3322785 | biostudies-literature
| S-EPMC4604376 | biostudies-literature
| S-EPMC3346618 | biostudies-literature
| S-EPMC162793 | biostudies-other
| S-EPMC3535987 | biostudies-literature
| S-EPMC2533458 | biostudies-literature
| S-EPMC8513173 | biostudies-literature