Ontology highlight
ABSTRACT:
SUBMITTER: Petrov A
PROVIDER: S-EPMC4826567 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Petrov Alexey A Grosely Rosslyn R Chen Jin J O'Leary Seán E SE Puglisi Joseph D JD
Molecular cell 20160401 1
The complexity of eukaryotic translation allows fine-tuned regulation of protein synthesis. Viruses use internal ribosome entry sites (IRESs) to minimize or, like the CrPV IRES, eliminate the need for initiation factors. Here, by exploiting the CrPV IRES, we observed the entire process of initiation and transition to elongation in real time. We directly tracked the CrPV IRES, 40S and 60S ribosomal subunits, and tRNA using single-molecule fluorescence spectroscopy and identified multiple parallel ...[more]