Dataset Information

Expression of two novel transcripts in the mouse definitive endoderm.

ABSTRACT: Here we describe the expression of two novel transcripts, Ende (AK014119) and Npe (AK084355), during early mouse embryogenesis. Ende mRNA was first detected at embryonic day (E) 7.0 in a small population of epiblast cells in the distal half of the embryo. At E7.5, Ende was expressed by newly formed definitive endoderm cells in the proximal half of the embryo, and was not expressed in extra-embryonic endoderm. This expression pattern then changed to the ventral aspect of the developing foregut pocket and the entire hindgut pocket at E8.0-8.5, before becoming restricted to the foregut overlying the heart and the posterior-most hindgut. By E9.25 Ende expression was also observed in the posterior half of the ventral neural tube. Thus, Ende was expressed dynamically and in specific populations of the definitive endoderm from E7.0 to E8.5. We found Npe expression to be restricted to the node/posterior notochord region at the distal tip of the embryo between E7.0 and E8.0. By E9.5, Npe expression was observed in the posterior-most population of dorsal hindgut cells and notochord cells. Given their expression in mouse definitive endoderm populations, Ende and Npe will be valuable tools to study formation and development of this tissue.

SUBMITTER: Hassan AS

PROVIDER: S-EPMC4850063 | biostudies-literature | 2010 Feb-Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Expression of two novel transcripts in the mouse definitive endoderm.

Hassan Ali S AS Hou Juan J Wei Wei W Hoodless Pamela A PA

Gene expression patterns : GEP 20100212 2-3

Here we describe the expression of two novel transcripts, Ende (AK014119) and Npe (AK084355), during early mouse embryogenesis. Ende mRNA was first detected at embryonic day (E) 7.0 in a small population of epiblast cells in the distal half of the embryo. At E7.5, Ende was expressed by newly formed definitive endoderm cells in the proximal half of the embryo, and was not expressed in extra-embryonic endoderm. This expression pattern then changed to the ventral aspect of the developing foregut po ...[more]

PMID: 20153842

Similar Datasets

Project description:We investigated the effects of hypoxia on spontaneous (SP)- and activin A (AA)-induced definitive endoderm (DE) differentiation of mouse embryonic stem cells (mESCs) and their subsequent differentiation into distal pulmonary epithelial cells. SP differentiation for 6 days of mESCs toward endoderm at hypoxia of 1% O2, but not at 3% or 21% (normoxia), increased the expression of Sox17 and Foxa2 by 31- and 63-fold above maintenance culture, respectively. Treatment of mESCs with 20 ng/mL AA for 6 days under hypoxia further increased the expression of DE marker genes Sox17, Foxa2, and Cxcr4 by 501-, 1,483-, and 126-fold above maintenance cultures, respectively. Transient exposure to hypoxia, as short as 24 h, was sufficient to enhance AA-induced endoderm formation. The involvement of hypoxia-inducible factor (HIF)-1α and reactive oxygen species (ROS) in the AA-induced endoderm enrichment was assessed using HIF-1α(-/-) mESCs and the ROS scavenger N-acetylcysteine (NAC). Under SP conditions, HIF-1α(-/-) mESCs failed to increase the expression of endodermal marker genes but rather shifted toward ectoderm. Hypoxia induced only a marginal potentiation of AA-induced endoderm differentiation in HIF-1α(-/-) mESCs. Treatment of mESCs with AA and NAC led to a dose-dependent decrease in Sox17 and Foxa2 expression. In addition, the duration of exposure to hypoxia in the course of a recently reported lung differentiation protocol resulted in differentially enhanced expression of distal lung epithelial cell marker genes aquaporin 5 (Aqp5), surfactant protein C (Sftpc), and secretoglobin 1a1 (Scgb1a1) for alveolar epithelium type I, type II, and club cells, respectively. Our study is the first to show the effects of in vitro hypoxia on efficient formation of DE and lung lineages. We suggest that the extent of hypoxia and careful timing may be important components of in vitro differentiation bioprocesses for the differential generation of distal lung epithelial cells from pluripotent progenitors.

Dataset Information

Expression of two novel transcripts in the mouse definitive endoderm.

Publications

Expression of two novel transcripts in the mouse definitive endoderm.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets