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Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.

ABSTRACT: A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromosomal loci.

SUBMITTER: Ma H

PROVIDER: S-EPMC4864854 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.

Ma Hanhui H Tu Li-Chun LC Naseri Ardalan A Huisman Maximiliaan M Zhang Shaojie S Grunwald David D Pederson Thoru T

Nature biotechnology 20160418 5

A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromo ...[more]

PMID: 27088723

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