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Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

ABSTRACT: Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

SUBMITTER: Myers SA

PROVIDER: S-EPMC6202184 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

Myers Samuel A SA Wright Jason J Peckner Ryan R Kalish Brian T BT Zhang Feng F Carr Steven A SA

Nature methods 20180507 6

Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts. ...[more]

PMID: 29735997

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