Ontology highlight
ABSTRACT:
SUBMITTER: Das S
PROVIDER: S-EPMC4890538 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Das Samir S Nag Arundhati A Liang JingXin J Bunck David N DN Umeda Aiko A Farrow Blake B Coppock Matthew B MB Sarkes Deborah A DA Finch Amethist S AS Agnew Heather D HD Pitram Suresh S Lai Bert B Yu Mary Beth MB Museth A Katrine AK Deyle Kaycie M KM Lepe Bianca B Rodriguez-Rivera Frances P FP McCarthy Amy A Alvarez-Villalonga Belen B Chen Ann A Heath John J Stratis-Cullum Dimitra N DN Heath James R JR
Angewandte Chemie (International ed. in English) 20150917 45
We describe a general synthetic strategy for developing high-affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope of interest is synthesized as a polypeptide, with a detection biotin tag and a strategically placed azide (or alkyne) presenting amino acid. This synthetic epitope (SynEp) is incubated with a library of complementary alkyne or azide presenting peptides. Library elements that bind the SynEp in the correct orientation undergo the Huisgen cyc ...[more]