Unknown

Dataset Information

0

Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.


ABSTRACT: Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.

SUBMITTER: Holtzer L 

PROVIDER: S-EPMC4919766 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.

Holtzer Laurent L   Oleinich Igor I   Anzola Marcello M   Lindberg Eric E   Sadhu Kalyan K KK   Gonzalez-Gaitan Marcos M   Winssinger Nicolas N  

ACS central science 20160518 6


Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living  ...[more]

Similar Datasets

| S-EPMC2891565 | biostudies-literature
| S-EPMC4291768 | biostudies-literature
| S-EPMC7726899 | biostudies-literature
| S-EPMC2774910 | biostudies-literature
| S-EPMC5508869 | biostudies-literature
| S-EPMC3531912 | biostudies-literature
| S-EPMC5504956 | biostudies-literature
| S-EPMC7473486 | biostudies-literature
| S-EPMC2748799 | biostudies-literature
| S-EPMC5873163 | biostudies-literature