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Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.


ABSTRACT: Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.

SUBMITTER: Holtzer L 

PROVIDER: S-EPMC4919766 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.

Holtzer Laurent L   Oleinich Igor I   Anzola Marcello M   Lindberg Eric E   Sadhu Kalyan K KK   Gonzalez-Gaitan Marcos M   Winssinger Nicolas N  

ACS central science 20160518 6


Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living  ...[more]

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