Ontology highlight
ABSTRACT:
SUBMITTER: Holtzer L
PROVIDER: S-EPMC4919766 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Holtzer Laurent L Oleinich Igor I Anzola Marcello M Lindberg Eric E Sadhu Kalyan K KK Gonzalez-Gaitan Marcos M Winssinger Nicolas N
ACS central science 20160518 6
Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living ...[more]