Nucleic Acid Templated Chemical Reaction in a Live Vertebrate.

ABSTRACT: Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.

SUBMITTER: Holtzer L 

PROVIDER: S-EPMC4919766 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature