Ontology highlight
ABSTRACT:
SUBMITTER: Crawford AM
PROVIDER: S-EPMC4928650 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Crawford Andrew M AM Kurecka Patrick P Yim Tsz Kwan TK Kozemchak Claire C Deb Aniruddha A Dostál Lubomír L Sun Cheng Jun CJ Brewe Dale L DL Barrea Raul R Penner-Hahn James E JE
Journal of synchrotron radiation 20160617 Pt 4
An X-ray fluorescence flow cytometer that can determine the total metal content of single cells has been developed. Capillary action or pressure was used to load cells into hydrophilic or hydrophobic capillaries, respectively. Once loaded, the cells were transported at a fixed vertical velocity past a focused X-ray beam. X-ray fluorescence was then used to determine the mass of metal in each cell. By making single-cell measurements, the population heterogeneity for metals in the µM to mM concent ...[more]