Ontology highlight
ABSTRACT:
SUBMITTER: Marsboom G
PROVIDER: S-EPMC4945388 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Marsboom Glenn G Zhang Guo-Fang GF Pohl-Avila Nicole N Zhang Yanmin Y Yuan Yang Y Kang Hojin H Hao Bo B Brunengraber Henri H Malik Asrar B AB Rehman Jalees J
Cell reports 20160623 2
The molecular mechanisms underlying the regulation of pluripotency by cellular metabolism in human embryonic stem cells (hESCs) are not fully understood. We found that high levels of glutamine metabolism are essential to prevent degradation of OCT4, a key transcription factor regulating hESC pluripotency. Glutamine withdrawal depletes the endogenous antioxidant glutathione (GSH), which results in the oxidation of OCT4 cysteine residues required for its DNA binding and enhanced OCT4 degradation. ...[more]