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The "Speedy" Synthesis of Atom-Specific (15)N Imino/Amido-Labeled RNA.

ABSTRACT: Although numerous reports on the synthesis of atom-specific (15)N-labeled nucleosides exist, fast and facile access to the corresponding phosphoramidites for RNA solid-phase synthesis is still lacking. This situation represents a severe bottleneck for NMR spectroscopic investigations on functional RNAs. Here, we present optimized procedures to speed up the synthesis of (15)N(1) adenosine and (15)N(1) guanosine amidites, which are the much needed counterparts of the more straightforward-to-achieve (15)N(3) uridine and (15)N(3) cytidine amidites in order to tap full potential of (1)H/(15)N/(15)N-COSY experiments for directly monitoring individual Watson-Crick base pairs in RNA. Demonstrated for two preQ1 riboswitch systems, we exemplify a versatile concept for individual base-pair labeling in the analysis of conformationally flexible RNAs when competing structures and conformational dynamics are encountered.

SUBMITTER: Neuner S

PROVIDER: S-EPMC4946632 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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The "Speedy" Synthesis of Atom-Specific (15)N Imino/Amido-Labeled RNA.

Neuner Sandro S Santner Tobias T Kreutz Christoph C Micura Ronald R

Chemistry (Weinheim an der Bergstrasse, Germany) 20150617 33

Although numerous reports on the synthesis of atom-specific (15)N-labeled nucleosides exist, fast and facile access to the corresponding phosphoramidites for RNA solid-phase synthesis is still lacking. This situation represents a severe bottleneck for NMR spectroscopic investigations on functional RNAs. Here, we present optimized procedures to speed up the synthesis of (15)N(1) adenosine and (15)N(1) guanosine amidites, which are the much needed counterparts of the more straightforward-to-achiev ...[more]

PMID: 26237536

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Dataset Information

The "Speedy" Synthesis of Atom-Specific (15)N Imino/Amido-Labeled RNA.

Publications

The "Speedy" Synthesis of Atom-Specific (15)N Imino/Amido-Labeled RNA.

Similar Datasets

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