Ontology highlight
ABSTRACT:
SUBMITTER: Wagner DT
PROVIDER: S-EPMC4948183 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Wagner Drew T DT Stevens D Cole DC Mehaffey M Rachel MR Manion Hannah R HR Taylor Richard E RE Brodbelt Jennifer S JS Keatinge-Clay Adrian T AT
Chemical communications (Cambridge, England) 20160701 57
C-methyltransferases (MTs) from modular polyketide synthase assembly lines are relatively rare and unexplored domains that are responsible for installing α-methyl groups into nascent polyketide backbones. The stage at which these synthase-embedded enzymes operate during polyketide biosynthesis has yet to be conclusively demonstrated. In this work we establish the activity and substrate preference for six MTs from the gephyronic acid polyketide synthase and demonstrate their ability to methylate ...[more]