Ontology highlight
ABSTRACT:
SUBMITTER: Skiba MA
PROVIDER: S-EPMC6470024 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Skiba Meredith A MA Bivins Marissa M MM Schultz John R JR Bernard Steffen M SM Fiers William D WD Dan Qingyun Q Kulkarni Sarang S Wipf Peter P Gerwick William H WH Sherman David H DH Aldrich Courtney C CC Smith Janet L JL
ACS chemical biology 20181203 12
Modular type I polyketide synthases (PKSs) produce some of the most chemically complex metabolites in nature through a series of multienzyme modules. Each module contains a variety of catalytic domains to selectively tailor the growing molecule. PKS O-methyltransferases ( O-MTs) are predicted to methylate β-hydroxyl or β-keto groups, but their activity and structure have not been reported. We determined the domain boundaries and characterized the catalytic activity and structure of the StiD and ...[more]