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Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.

ABSTRACT: In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.

SUBMITTER: Gao R

PROVIDER: S-EPMC4962057 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.

Gao Rong R Yu Kai K Nie Jukui J Lian Tengfei T Jin Jianshi J Liljas Anders A Su Xiao-Dong XD

Scientific reports 20160727

In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation r ...[more]

PMID: 27460773

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Deep sequencing reveals mRNA recruitment in translation initiation

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