Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis.
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ABSTRACT:
SUBMITTER: Wells AL
PROVIDER: S-EPMC4963622 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Publications
Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis.
Wells Amber L AL Condeelis John S JS Singer Robert H RH Zenklusen Daniel D
CSH protocols 20071101
INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced. ...[more]
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