Ontology highlight
ABSTRACT:
SUBMITTER: Xie Q
PROVIDER: S-EPMC4966620 | biostudies-literature | 2016 Feb-Mar
REPOSITORIES: biostudies-literature
Xie Qing Q Moore Benjamin B Beardsley Richard L RL
mAbs 20151214 2
Tryptic peptide mapping analysis of a Chinese hamster ovary (CHO)-expressed, recombinant IgG1 monoclonal antibody revealed a previously unreported +16 Da modification. Through a combination of MS(n) experiments, and preparation and analysis of known synthetic peptides, the possibility of a sequence variant (Ala to Ser) was ruled out and the presence of hydroxylysine was confirmed. Post-translational hydroxylation of lysine was found in a consensus sequence (XKG) known to be the site of modificat ...[more]