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Topology of chromosome centromeres in human sperm nuclei with high levels of DNA damage.


ABSTRACT: Several studies have shown that the 'poor' sperm DNA quality appears to be an important factor affecting male reproductive ability. In the case of sperm cells from males with the correct somatic karyotype but with deficient spermatogenesis, resulting in a high degree of sperm DNA fragmentation, we observed changes in the preferential topology of the chromosome 7, 9, 15, 18, X and Y centromeres. The changes occurred in radial localization and may have been directly linked to the sperm chromatin damage. This conclusion is mainly based on a comparison of FISH signals that were observed simultaneously in the TUNEL-positive and TUNEL-negative sperm cells. The analyzed cells originated from the same ejaculated sample and FISH was performed on the same slides, after in situ TUNEL reaction. Based on the observed changes and previous data, it appears that the sperm nucleus architecture can be disrupted by a variety of factors and has a negative influence on spermatogenesis at the same time. Often, these factors coexist (e.g. chromosomal translocations, aneuploidies, a higher DNA fragmentation, abnormal seminology), but no direct correlations between the factors were observed.

SUBMITTER: Wiland E 

PROVIDER: S-EPMC4997348 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Voigt effect-based wide-field magneto-optical microscope integrated in a pump-probe experimental setup.

Janda T T   Nádvorník L L   Kuchařík J J   Butkovičová D D   Schmoranzerová E E   Trojánek F F   Němec P P  

The Review of scientific instruments 20180701 7


In this work, we describe an experimental setup for a spatially resolved pump-probe experiment with an integrated wide-field magneto-optical (MO) microscope. The MO microscope can be used to study ferromagnetic materials with both perpendicular-to-plane and in-plane magnetic anisotropy via polar Kerr and Voigt effects, respectively. The functionality of the Voigt effect-based microscope was tested using an in-plane magnetized ferromagnetic semiconductor (Ga,Mn)As. It was revealed that the presen  ...[more]