Ontology highlight
ABSTRACT:
SUBMITTER: Ma H
PROVIDER: S-EPMC5004447 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Ma Hanhui H Tu Li-Chun LC Naseri Ardalan A Huisman Maximiliaan M Zhang Shaojie S Grunwald David D Pederson Thoru T
The Journal of cell biology 20160822 5
The bacterial CRISPR-Cas9 system has been repurposed for genome engineering, transcription modulation, and chromosome imaging in eukaryotic cells. However, the nuclear dynamics of clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) guide RNAs and target interrogation are not well defined in living cells. Here, we deployed a dual-color CRISPR system to directly measure the stability of both Cas9 and guide RNA. We found that Cas9 is essential for guide RN ...[more]