Ontology highlight
ABSTRACT:
SUBMITTER: Aouida M
PROVIDER: S-EPMC5801905 | biostudies-other | 2016 Dec
REPOSITORIES: biostudies-other
Aouida Mustapha M Eid Ayman A Mahfouz Magdy M MM
Biochimie open 20160224
CRISPR/Cas9 system confers molecular immunity in archeal and bacterial species against invading foreign nucleic acids. CRISPR/Cas9 system is used for genome engineering applications across diverse eukaryotic species. In this study, we demonstrate the utility of the CRISPR/Cas9 genome engineering system for drug target validation in human cells. Pladienolide B is a natural macrolide with antitumor activities mediated through the inhibition of pre-mRNA splicing. To validate the spliceosomal target ...[more]