Sirtuin 6 (SIRT6) Activity Assays.
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ABSTRACT:
SUBMITTER: Rahnasto-Rilla M
PROVIDER: S-EPMC5004593 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
Sirtuin 6 (SIRT6) Activity Assays.
Rahnasto-Rilla Minna M Lahtela-Kakkonen Maija M Moaddel Ruin R
Methods in molecular biology (Clifton, N.J.) 20160101
SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as ...[more]
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