Ontology highlight
ABSTRACT:
SUBMITTER: Vaid R
PROVIDER: S-EPMC5005230 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Vaid Rajni R Dev Kamal K Lichten Michael M Sourirajan Anuradha A
3 Biotech 20160830 2
Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-like kinases (PLK) are serine/threonine protein kinases that are the crucial regulators of cell cycle. Cdc5, the solitary PLK in budding yeast Saccharomyces cerevisiae, has diverse array of targets in ce ...[more]