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Photoswitchable Spiropyran Dyads for Biological Imaging.

ABSTRACT: The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photochromic spironaphthothiopyran unit, which serves as a photoswitchable quencher, is reported. This system can be switched reversibly between the fluorescent and nonfluorescent states using visible light at wavelengths of 405 and 630 nm, respectively, and it works effectively in aqueous solution. Live-cell imaging demonstrates that this dyad has several desirable features, including excellent membrane permeability, fast and reversible modulation of fluorescence by visible light, and good contrast between the bright and dark states.

SUBMITTER: Xiong Y

PROVIDER: S-EPMC5010358 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Photoswitchable Spiropyran Dyads for Biological Imaging.

Xiong Yaoyao Y Rivera-Fuentes Pablo P Sezgin Erdinc E Vargas Jentzsch Andreas A Eggeling Christian C Anderson Harry L HL

Organic letters 20160726 15

The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photochromic spironaphthothiopyran unit, which serves as a photoswitchable quencher, is reported. This system can be switched reversibly between the fluorescent and nonfluorescent states using visible light at wavelengths of 405 and 630 nm, respectively, and it works effectively in aqueous solution. Live-cell imaging demonstrates that this dyad has several desirable featur ...[more]

PMID: 27456166

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