Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo.

ABSTRACT: Mitosis is critical for organismal growth and differentiation. The process is highly dynamic and requires ordered events to accomplish proper chromatin condensation, microtubule-kinetochore attachment, chromosome segregation, and cytokinesis in a small time frame. Errors in the delicate process can result in human disease, including birth defects and cancer. Traditional approaches investigating human mitotic disease states often rely on cell culture systems, which lack the natural physiology and developmental/tissue-specific context advantageous when studying human disease. This protocol overcomes many obstacles by providing a way to visualize, with high resolution, chromosome dynamics in a vertebrate system, the zebrafish. This protocol will detail an approach that can be used to obtain d

SUBMITTER: Percival SM 

PROVIDER: S-EPMC6082026 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature