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Quantitative high-throughput population dynamics in continuous-culture by automated microscopy.

ABSTRACT: We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

SUBMITTER: Merritt J 

PROVIDER: S-EPMC5018735 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Quantitative high-throughput population dynamics in continuous-culture by automated microscopy.

Merritt Jason J   Kuehn Seppe S  

Scientific reports 20160912

We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation. ...[more]

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