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High-Throughput Automated Microscopy of Circulating Tumor Cells.

ABSTRACT: Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures. An excellent agreement between our machine vision-based approach and a state-of-the-art microscopy equipment was achieved. Our integral approach provides a path for operator-free and robust analysis of cancer cells as a standard clinical practice.

SUBMITTER: Aguilar-Avelar C

PROVIDER: S-EPMC6760523 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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High-Throughput Automated Microscopy of Circulating Tumor Cells.

Aguilar-Avelar Carlos C Soto-García Brenda B Aráiz-Hernández Diana D Yee-de León Juan F JF Esparza Miguel M Chacón Franco F Delgado-Balderas Jesús Rolando JR Alvarez Mario M MM Trujillo-de Santiago Grissel G Gómez-Guerra Lauro S LS Velarde-Calvillo Liza P LP Abarca-Blanco Alejandro A Wong-Campos J D JD

Scientific reports 20190924 1

Circulating tumor cells (CTCs) have the potential of becoming the gold standard marker for cancer diagnosis, prognosis and monitoring. However, current methods for its isolation and characterization suffer from equipment variability and human operator error that hinder its widespread use. Here we report the design and construction of a fully automated high-throughput fluorescence microscope that enables the imaging and classification of cancer cells that were labeled by immunostaining procedures ...[more]

PMID: 31551445

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