Ontology highlight
ABSTRACT:
SUBMITTER: Genshaft AS
PROVIDER: S-EPMC5027636 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Genshaft Alex S AS Li Shuqiang S Gallant Caroline J CJ Darmanis Spyros S Prakadan Sanjay M SM Ziegler Carly G K CG Lundberg Martin M Fredriksson Simon S Hong Joyce J Regev Aviv A Livak Kenneth J KJ Landegren Ulf U Shalek Alex K AK
Genome biology 20160919 1
We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidigm C1™ system, we profile the transcriptomic and proteomic response of a human breast adenocarcinoma cell line to a chemical perturbation, benchmarking against in situ hybridizations and immunofluoresc ...[more]