Ontology highlight
ABSTRACT:
SUBMITTER: Lee J
PROVIDER: S-EPMC5043417 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Lee Joongoo J Boersma Arnold J AJ Boudreau Marc A MA Cheley Stephen S Daltrop Oliver O Li Jianwei J Tamagaki Hiroko H Bayley Hagan H
ACS nano 20160830 9
Protein engineering has been used to remodel pores for applications in biotechnology. For example, the heptameric α-hemolysin pore (αHL) has been engineered to form a nanoreactor to study covalent chemistry at the single-molecule level. Previous work has been confined largely to the chemistry of cysteine side chains or, in one instance, to an irreversible reaction of an unnatural amino acid side chain bearing a terminal alkyne. Here, we present four different αHL pores obtained by coupling eithe ...[more]