Unknown

Dataset Information

0

Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15.


ABSTRACT: The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions. DES-15 secreted nearly 50 % more protein than RUT C30. The gene expression level of a set of genes (cla4, spa2, ras2, ras1, rhoA, cdc42, and racA) known to be involved in hyphae growth and hyphal branching was measured by quantitative real-time PCR. The transcriptional analysis of these genes demonstrated that a decrease in gene expressions might contribute to the increased hyphal branching seen in DES-15. These results indicated that the highly branching hyphae in DES-15 resulted in increased cellulase production, suggesting that DES-15 may be a good candidate for use in the large-scale production of cellulase.

SUBMITTER: He R 

PROVIDER: S-EPMC5052222 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15.

He Ronglin R   Li Chen C   Ma Lijuan L   Zhang Dongyuan D   Chen Shulin S  

3 Biotech 20161005 2


The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of  ...[more]

Similar Datasets

| S-EPMC9113457 | biostudies-literature
| S-EPMC7101855 | biostudies-literature
| S-EPMC3091638 | biostudies-literature
| S-EPMC5956553 | biostudies-literature
| S-EPMC6240962 | biostudies-literature
| S-EPMC4528718 | biostudies-literature
| S-EPMC3370264 | biostudies-literature
| S-EPMC5865335 | biostudies-literature
| S-EPMC152388 | biostudies-literature
| S-EPMC7110754 | biostudies-literature