Ontology highlight
ABSTRACT:
SUBMITTER: Mika JT
PROVIDER: S-EPMC5052448 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Mika Jacek T JT Thompson Alexander J AJ Dent Michael R MR Brooks Nicholas J NJ Michiels Jan J Hofkens Johan J Kuimova Marina K MK
Biophysical journal 20161001 7
The viscosity is a highly important parameter within the cell membrane, affecting the diffusion of small molecules and, hence, controlling the rates of intracellular reactions. There is significant interest in the direct, quantitative assessment of membrane viscosity. Here we report the use of fluorescence lifetime imaging microscopy of the molecular rotor BODIPY C10 in the membranes of live Escherichia coli bacteria to permit direct quantification of the viscosity. Using this approach, we inves ...[more]