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ABSTRACT:
SUBMITTER: Vysniauskas A
PROVIDER: S-EPMC5520772 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Vyšniauskas Aurimas A Qurashi Maryam M Gallop Nathaniel N Balaz Milan M Anderson Harry L HL Kuimova Marina K MK
Chemical science 20150706 10
Viscosity and temperature variations in the microscopic world are of paramount importance for diffusion and reactions. Consequently, a plethora of fluorescent probes have evolved over the years to enable fluorescent imaging of both parameters in biological cells. However, the simultaneous effect of both temperature and viscosity on the photophysical behavior of fluorophores is rarely considered, yet unavoidable variations in temperature can lead to significant errors in the readout of viscosity ...[more]