Ontology highlight
ABSTRACT:
SUBMITTER: Shimolina LE
PROVIDER: S-EPMC5278387 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Shimolina Lyubov' E LE Izquierdo Maria Angeles MA López-Duarte Ismael I Bull James A JA Shirmanova Marina V MV Klapshina Larisa G LG Zagaynova Elena V EV Kuimova Marina K MK
Scientific reports 20170130
The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic v ...[more]