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Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum-mitochondria tether.


ABSTRACT: The discovery of the multiple roles of mitochondria-endoplasmic reticulum (ER) juxtaposition in cell biology often relied upon the exploitation of Mitofusin (Mfn) 2 as an ER-mitochondria tether. However, this established Mfn2 function was recently questioned, calling for a critical re-evaluation of Mfn2's role in ER-mitochondria cross-talk. Electron microscopy and fluorescence-based probes of organelle proximity confirmed that ER-mitochondria juxtaposition was reduced by constitutive or acute Mfn2 deletion. Functionally, mitochondrial uptake of Ca2+ released from the ER was reduced following acute Mfn2 ablation, as well as in Mfn2-/- cells overexpressing the mitochondrial calcium uniporter. Mitochondrial Ca2+ uptake rate and extent were normal in isolated Mfn2-/- liver mitochondria, consistent with the finding that acute or chronic Mfn2 ablation or overexpression did not alter mitochondrial calcium uniporter complex component levels. Hence, Mfn2 stands as a bona fide ER-mitochondria tether whose ablation decreases interorganellar juxtaposition and communication.

SUBMITTER: Naon D 

PROVIDER: S-EPMC5056088 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum-mitochondria tether.

Naon Deborah D   Zaninello Marta M   Giacomello Marta M   Varanita Tatiana T   Grespi Francesca F   Lakshminaranayan Sowmya S   Serafini Annalisa A   Semenzato Martina M   Herkenne Stephanie S   Hernández-Alvarez Maria Isabel MI   Zorzano Antonio A   De Stefani Diego D   Dorn Gerald W GW   Scorrano Luca L  

Proceedings of the National Academy of Sciences of the United States of America 20160919 40


The discovery of the multiple roles of mitochondria-endoplasmic reticulum (ER) juxtaposition in cell biology often relied upon the exploitation of Mitofusin (Mfn) 2 as an ER-mitochondria tether. However, this established Mfn2 function was recently questioned, calling for a critical re-evaluation of Mfn2's role in ER-mitochondria cross-talk. Electron microscopy and fluorescence-based probes of organelle proximity confirmed that ER-mitochondria juxtaposition was reduced by constitutive or acute Mf  ...[more]

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